Description
Intended Uses: The Astrovirus ELISA is an enzyme immunoassay for the measurement of Astrovirus in faecal samples. For Research Use Only.
Principle of the Assay: The Astrovirus is a one-step enzyme immunoassay on the basis of polyclonal and monoclonal antibodies against Astrovirus antigens. Diluted stool specimens and horseradish peroxidase (HRP) labelled monoclonal anti-Astrovirus-antibodies are dispensed simultaneously into the wells of a microtitration plate coated with polyclonal anti-Astrovirus-antibodies. After an incubation time of 60 min at room temperature (RT) unbound components are removed by a washing step. HRP converts the subsequently added colourless substrate solution of 3,3',5,5'-Tetramethylbenzidine (TMB) within a 10 min reaction time at room temperature protected from light into a blue product. The enzyme reaction is terminated by sulphuric acid dispensed into the wells turning the solution from blue to yellow. The optical density (OD) of the solution read at 450 / >= 620 nm is directly proportional to the specifically bound amount of Astrovirus: